Background and Objective: The sludge from biogas production can be used as organic fertilizer and carrier agent of Trichoderma harzianum which functions as plant growth stimulator and bio-fungicide. This research aimed to determine the effect of different volumes of Trichoderma harzianum inoculum on the concentration of nutrients and organic fertilizer as carrier agent of Trichoderma harzianum. Methodology: Rice husk charcoal and Trichoderma harzianum inoculum in broken corn were added to the sludge. This research applied four treatments with the addition of different volumes of inoculum, and do repetitions of each treatment three times. Testing the ability of the mixture in storing Trichoderma harzianum tests eight times once a week. Testing the ability of Trichoderma harzianum in decomposing organic materials until they are ready to use the test be repeated three times. The variables observed were the total volume of nitrogen phosphorus kalium and conidiophores. Data obtained were examined by ANOVA analysis and conducted the Duncan test if there are differences between treatments. Results: The result showed that sludge could not yet be used as organic fertilizer since the result was below 4. The addition of inoculum did not guarantee the higher amount of living conidiophores. On the contrary, the less inoculum was given the more conidia could survive especially in the fourth and fifth week. The mixture could keep Trichoderma harzianum optimally until the sixth week since it was incubated. The result of in vivo test to mustard greens plant the result showed no significant difference. Conclusion: The conclusion of the research is the increase in concentration nutrient (N, P, and K), and the formula which had the highest average value was T3 (with the addition of 112.5 g of Trichoderma harzianum). Every time a number of conidia/conidiophores in each treatment was measured, the results fluctuated. In vivo test showed no significant result from all given formulas.