Background and Objective: The biogas sludge is generally not used optimally and people even pour it directly into the river that it cause environmental pollution. One of the ways to get the benefit from sludge and chicken blood meal or fishmeal is to use it as a substitute for bran in mushroom media. This study aimed to improve the nutrient content of biogas sludge with the addition of chicken blood meal (CBM) and fishmeal (FM) as substitute material for bran in white oyster mushroom media. Materials and Methods: The biogas sludge was dried in the sun for 3 days until its form resembled the soil. The treatment consisted of dried biogas sludge without CBM (BP0), dried biogas sludge with 1% CBM (BP1) and dried biogas sludge with 3% CBM (BP2). Each was added to the media of white oyster mushroom as much as 15% as substitute material for bran. The other treatment consisted of dried biogas sludge without FM (FP0), dried biogas sludge with 2% FM (FP1) and dried biogas sludge with 4% FM (FP2). Each was added to the media of white oyster mushroom as much as 5% as substitute material for bran. BP0 and FP0 created from white oyster mushroom media were commonly used by farmers. Each treatment was analyzed of nutritional and biological contents. All the data were tested using completely randomized design one-way ANOVA. Results: The results of the research on the use of chicken blood meal and fishmeal showed that the best treatments were BP2 and FP2. Their nutrient content increased, including the organic-C, organic matter, nitrogen, P2O5 and K2O. The productivity of the oyster mushroom increased, shown by the increase of fresh weight and diameter of caps in treatment BP2. The replacement of bran by biogas sludge with 4% FM addition (FP2) in oyster mushroom media increased the fresh weight, the number of caps and the length of oyster mushroom stalks. Conclusion: The best treatments for mushroom media were BP2 and FP2 to be used as substitute material for bran in white oyster mushroom media.